The T7EI calculator is designed to be used in conjuction with the Gel Analyzer 2010a software tool, which allows researchers to perform a semi-automated analysis of T7EI mismatch detection gels. The researcher should use the GelAnalyzer 2010a software tool to detect lanes/bands and calculate the raw volumes for each of their samples, then input the resulting Analysis info table into this calculator to determine the percent gene editing for each lane. For more information on how to generate the Analysis info table, please follow these instructions: Show SOP